@Override protected void makeColumns(Run run, Partition partition, PoolableElementView dilution, String userName, String[] output) { output[0] = dilution.getLibraryName(); output[1] = dilution.getLibraryAlias(); final Optional<Index> firstIndex = dilution.getIndices().stream().filter(i -> i.getPosition() == 1).findFirst(); output[2] = firstIndex.map(Index::getName).orElse(""); output[3] = firstIndex.map(Index::getSequence).orElse(""); final Optional<Index> secondIndex = dilution.getIndices().stream().filter(i -> i.getPosition() == 2).findFirst(); output[4] = secondIndex.map(Index::getName).orElse(""); output[5] = secondIndex.map(Index::getSequence) .map(run.getSequencer().getInstrumentModel().getDataManglingPolicy() == InstrumentDataManglingPolicy.I5_RC ? SampleSheet::reverseComplement : Function.identity()) .orElse(""); }
InstrumentPosition position = null; if (!isStringEmptyOrNull(positionName)) { position = target.getSequencer().getInstrumentModel().getPositions().stream() .filter(pos -> positionName.equals(pos.getAlias())) .findFirst().orElseThrow(() -> new IllegalArgumentException(String.format("Unknown position '%s' for platform '%s'", positionName, target.getSequencer().getInstrumentModel().getAlias())));
private void applyChanges(Run target, Run source) throws IOException { validateChanges(target, source); target.setAlias(source.getAlias()); target.setAccession(source.getAccession()); target.setDescription(source.getDescription()); target.setFilePath(source.getFilePath()); target.setHealth(source.getHealth()); target.setStartDate(source.getStartDate()); target.setCompletionDate(source.getCompletionDate()); target.setMetrics(source.getMetrics()); makeContainerChangesChangeLog(target, target.getSequencerPartitionContainers(), source.getSequencerPartitionContainers()); applyContainerChanges(target, source); target.setSequencingParameters(source.getSequencingParameters()); target.setSequencer(source.getSequencer()); if (isIlluminaRun(target)) { applyIlluminaChanges((IlluminaRun) target, (IlluminaRun) source); } else if (isLS454Run(target)) { applyLS454Changes((LS454Run) target, (LS454Run) source); } else if (isSolidRun(target)) { applySolidChanges((SolidRun) target, (SolidRun) source); } else if (isOxfordNanoporeRun(target)) { applyOxfordNanoporeChanges((OxfordNanoporeRun) target, (OxfordNanoporeRun) source); } }
@Override protected void makeColumns(Run run, Partition p, PoolableElementView dilution, String userName, String[] output) { output[0] = p.getSequencerPartitionContainer().getIdentificationBarcode(); output[1] = p.getPartitionNumber().toString(); output[2] = String.format("%d_%s_%s", p.getSequencerPartitionContainer().getId(), dilution.getLibraryName(), dilution.getDilutionName()); output[3] = dilution.getSampleAlias().replaceAll("\\s", ""); output[4] = dilution.getIndices().stream()// .sorted(Comparator.comparingInt(Index::getPosition))// .map(i -> { if (run.getSequencer().getInstrumentModel().getDataManglingPolicy() == InstrumentDataManglingPolicy.I5_RC && i.getPosition() == 2) { return reverseComplement(i.getSequence()); } return i.getSequence(); })// .collect(Collectors.joining("-")); output[5] = dilution.getLibraryDescription(); output[6] = "N"; output[7] = "NA"; output[8] = userName; }
if (from.getSequencer() != null) { dto.setPlatformType(from.getSequencer().getInstrumentModel().getPlatformType().getKey()); } else { dto.setPlatformType("");
private void validateChanges(Run before, Run changed) throws IOException { List<ValidationError> errors = new ArrayList<>(); if (!changed.getHealth().isDone()) { changed.setCompletionDate(null); } else if (changed.getCompletionDate() == null) { errors.add(new ValidationError("completionDate", "Completion date must be provided for finished run")); } if (before != null) { if (before.getCompletionDate() != null && changed.getCompletionDate() != null && !changed.getCompletionDate().equals(before.getCompletionDate()) && !authorizationManager.isAdminUser()) { errors.add(new ValidationError("completionDate", "Only admin may change completion date")); } if (before.getStartDate() != null && changed.getStartDate() != null && !changed.getStartDate().equals(before.getStartDate()) && !authorizationManager.isAdminUser()) { errors.add(new ValidationError("completionDate", "Only admin may change start date")); } } InstrumentModel platform = changed.getSequencer().getInstrumentModel(); for (RunPosition position : changed.getRunPositions()) { if (position.getPosition() != null && !platform.getPositions().contains(position.getPosition())) { errors.add(new ValidationError( String.format("Platform %s does not have a position %s", platform.getAlias(), position.getPosition()))); } } if (!errors.isEmpty()) { throw new ValidationException(errors); } }
/** * Loads persisted objects into run fields. Should be called before saving or updating. Loads all fields except for: * <ul> * <li>creator/lastModifier User objects</li> * </ul> * * @param run the Run to load entities into. Must contain at least the IDs of the objects to load. * @throws IOException */ private void loadChildEntities(Run run) throws IOException { if (run.getSequencingParameters() != null) { run.setSequencingParameters(sequencingParametersService.get(run.getSequencingParameters().getId())); } run.setSequencer(instrumentService.get(run.getSequencer().getId())); }
public static ContainerDto asDto(@Nonnull SequencerPartitionContainer from, boolean includePartitions, boolean includePoolContents) { ContainerDto dto = new ContainerDto(); dto.setId(from.getId()); dto.setIdentificationBarcode(from.getIdentificationBarcode()); dto.setModel(asDto(from.getModel())); Run lastRun = from.getLastRun(); if (lastRun != null) { dto.setLastRunAlias(lastRun.getAlias()); dto.setLastRunId(lastRun.getId()); dto.setLastSequencerId(lastRun.getSequencer().getId()); dto.setLastSequencerName(lastRun.getSequencer().getName()); } if (from.getLastModified() != null) { dto.setLastModified(formatDateTime(from.getLastModified())); } if (from.getClusteringKit() != null) { dto.setClusteringKit(asDto(from.getClusteringKit())); } if (from.getMultiplexingKit() != null) { dto.setMultiplexingKit(asDto(from.getMultiplexingKit())); } if (includePartitions) { dto.setPartitions(asPartitionDtos(from.getPartitions(), includePoolContents)); } return dto; }